Fig. 1: Characterization of the MD65 Ab versions. | Nature Communications

Fig. 1: Characterization of the MD65 Ab versions.

From: Post-exposure protection of SARS-CoV-2 lethal infected K18-hACE2 transgenic mice by neutralizing human monoclonal antibody

Fig. 1

a Binding profiles of MD65 Ab variants (specified by different colors as indicated in the respective inset legends) tested by ELISA against S1. Values along the curve depict averages of triplicates ± SEM. b SARS-CoV-2 in vitro neutralization potency of the MD65 Ab variants evaluated by plaque reduction neutralization test (PRNT). Values are averages of triplicates ± SEM. c BLI-determined binding of hACE2 to RBD in the presence of MD65 Ab variants or MD29 IgG-YTE (as a control). Each of the biotinylated antibodies was immobilized on a streptavidin sensor, saturated with RBD, washed, and incubated with recombinant hACE2 for 180 s. Time 0 represents the binding of the hACE2 to the antibody–RBD complex. The MD65 IgG curve completely overlapped with and is therefore masked by the MD65 IgG-YTE.

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