Fig. 4: Viral load determination and histological analysis of lungs, collected from K18-hACE2 mice, infected with SARS-CoV-2, and treated 2 dpi with MD65 antibody. | Nature Communications

Fig. 4: Viral load determination and histological analysis of lungs, collected from K18-hACE2 mice, infected with SARS-CoV-2, and treated 2 dpi with MD65 antibody.

From: Post-exposure protection of SARS-CoV-2 lethal infected K18-hACE2 transgenic mice by neutralizing human monoclonal antibody

Fig. 4

a, b Viral load in lung samples, collected 6 dpi from PBS-treated mice (n = 6, gray dots) and mice treated 2 dpi with 1 mg MD65 antibody (n = 6, turquoise dots). a Viral load was quantified by qRT-PCR and expressed as equivalents of PFU/ml. b Infectious viral load determined by plaque assay. Dotted red line indicates the assay limit of detection. Data in a, b represent individual values and mean ± SEM. Horizontal bars indicate statistical significance of paired values. P values calculated using two-tailed paired t-test were: in panel a—0.0295 and in panel b—0.0031. c, d Histological analysis of lung sections, collected 21 dpi from a naïve mouse (c) and a mouse that was infected with SARS-CoV-2 and treated 2 dpi with 1 mg MD65 antibody (d); black arrow indicates lymphoid aggregate. The panels include representative images (n = 2 for the naïve mice and n = 6 for the MD65-treated mice, which were independently infected and analyzed, yielding similar results; full images of the lungs are provided in Supplementary Fig. 3). Magnification = ×40.

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