Fig. 5: IL-10R blocking activates a cell-to-cell communication module in LPS-stimulated DCs.
From: Dissection of intercellular communication using the transcriptome-based framework ICELLNET
a Depicted are the four experimental conditions for which transcriptomics was generated (n = 6 biologically independent samples). b Connectivity maps describing outward communication from DCs to putative target cells in the conditions: Medium (M), LPS (L), LPS + aTNFR and LPS + aIL-10R. Twelve primary cell types are considered as partner cells (DC: conventional dendritic cell, Macroph: macrophage, Mono: monocyte, Ostblast: osteoblast, Endoth: endothelial cell, Fblast: fibroblast, Kerat: keratinocyte, Epith: epithelial cell, B cell, T cell, Neutrop: neutrophil and NK: natural killer cell) and are colored depending on the cell compartment (green: stroma, orange: innate, blue: adaptive, pink: epithelium). The width of the edges corresponds to a global score combining the intensity of all the individual ligand/receptor interactions, normalized to the medium condition. A scale ranging from 1 to 10, corresponding to minimum and maximum communication scores, is shown in the legend. c Gene corresponding to ligands (black) and receptors (white) counted in each loop signature (from n = 6 biologically independent samples for each conditions) and plotted according to regulation directionality: upregulated (Up) or downregulated (Down). Genes with separability score ≥4 were included in each condition’s signature. d Balloon plot representing a selection of individual interaction scores. e Protein levels of IL-6 (n = 10 biologically independent samples, except for Medium condition where n = 5), OSM (n = 4 biologically independent samples), IL-23 (n = 8 biologically independent samples) and IL-12p70 (n = 16 biologically independent samples, except for Medium condition where n = 8) demonstrating increased secretion in LPS + aIL-10R DC supernatant. Data are presented as mean values ± SEM. Some schematic art pieces were used and modified from Servier Medical Art, licensed under a Creative Common Attribution 3.0 Generic License. http://smart.servier.com/ (a).
