Fig. 4: The corticostriatal projection to the tail of the striatum (TS) mediates crescendo-induced freezing.
From: Corticostriatal control of defense behavior in mice induced by auditory looming cues
a Left, injection of AAV-hSyn-eNpHR3.0 in TS. Right, eNpHR3.0 (green) expression in TS. Scale bar, 200 µm. LA lateral amygdala. b Behavioral responses to crescendo stimuli without (left) and with (right) optogenetic silencing of TS (n = 10 mice). c Comparison of crescendo-induced freezing duration (left, ***p = 1.061 × 10−4, two-sided paired t test, t = 5.962, n = 10 mice), normalized top speed (middle, p = 0.981, two-sided paired t test, t = −0.024) and escape latency (right, ***p = 3.800 × 10−5, two-sided paired t test, t = 6.835) without (LEDoff) and with (LEDon) silencing of TS. N.S., not significant. d Left, first injection of AAV1-Cre in AC and second injection of AAV1-DIO-ChR2 in TS. Right, expression of ChR2 in AC-recipient TS neurons. Scale bar, 200 µm. e Behavioral responses to crescendo stimuli without (left) and with (right) optogenetic activation of AC-recipient TS neurons (n = 8 mice). f Comparison of crescendo-induced freezing duration (left, ***p = 0.006, two-sided paired t test, t = −3.380, n = 8 mice), normalized top speed (middle, ***p = 0.001, two-sided paired t test, t = 5.316) and nest time (right, ***p = 1.628 × 10−5, two-sided paired t test, t = 9.381) without (LEDoff) and with (LEDon) coupling the activation of AC-recipient TS neurons.
