Fig. 3: Metabolic profile and chemical structures of the characterized compounds from heterologous expression of five of the seven positive uncharacterized BGCs.
From: Cas12a-assisted precise targeted cloning using in vivo Cre-lox recombination
a HPLC analysis of the crude extract from heterologous expression of BGC #1 from Streptomyces sp. NRRL F-6131 in S. avermitilis SUKA17 and the chemical structures for the 6 heterodimers (1–6). b HPLC analysis of the crude extract from heterologous expression of BGC #11 from Streptomyces alni NRRL B-24611 in S. avermitilis SUKA17 and the chemical structure for the 4 new angucyclines and derivatives (7–10). c Chemical structures of the rest of characterized compounds including two new anthraquinone or naphthoquinone derivatives (11 and 12) containing an acetylcysteine moiety identified from Streptomyces cyaneofuscatus NRRL B-2570 BGC #2, two molecules allenomycin A and allenomycin B (16 and 17) identified from Streptomyces griseofuscus NRRL B-5429 BGC #25, two known citreodiols (14 and 15) and a new derivative (13) identified from S. griseofuscus NRRL B-5429 BGC #38.
