Table 1 Summary of the results from the 43 uncharacterized BGCs cloned by CAPTURE.
From: Cas12a-assisted precise targeted cloning using in vivo Cre-lox recombination
Target BGC | Size | Predicted BGC type | Cloning efficiency | HPLC |
|---|---|---|---|---|
Streptomyces noursei ATCC 11455 | ||||
C29 | 26 kb | Lanthipeptide | 100% | No new peak |
C33 | 76 kb | NRPS | 100% | No new peak |
Streptomyces incarnatus NRRL 8089 | ||||
C5 | 13 kb | Siderophore | 100% | No new peak |
Streptomyces leeuwenhoekii NRRL B-24963 | ||||
C19 | 22 kb | Lassopeptide | 100% | No new peak |
Streptomyces griseochromogenes ATCC 14511 | ||||
C6 | 59 kb | NRPS, type I PKS | 100% | 1 new peak |
C7 | 113 kb | type I PKS, NRPS | 100% (4 colonies) | No new peak |
C11 | 24 kb | Lassopeptide | 100% | No new peak |
C15 | 11 kb | Siderophore | 100% | No new peak |
C21 | 100 kb | NRPS, type I PKS, Lanthipeptide | 86% (12 colonies) | No new peak |
C31 | 20 kb | Lassopeptide | 100% | No new peak |
C39 | 103 kb | NRPS, type III PKS | 100% (10 colonies) | No new peak |
C41 | 45 kb | type I PKS | 86% | No new peak |
Streptomyces griseoflavus NRRL B-1830 | ||||
C28 | 65 kb | NRPS-like, type I PKS | 100% | No new peak |
Streptomyces sp. NRRL B-11253 | ||||
C7 | 55 kb | Terpene-arylpolyene | 100% | No new peak |
Streptomyces sp. NRRL F-5635 | ||||
C10 | 72 kb | type II PKS | 100% | No new peak |
Streptomyces sp. NRRL F-6131 | ||||
C1 | 40 kb | type II PKS | 100% | 6 new peaks |
C4 | 50 kb | NRPS | 100% | No new peak |
C8 | 73 kb | type I PKS-NRPS | 100% | No new peak |
C9 | 51 kb | NRPS | 100% | No new peak |
C11 | 46 kb | type II PKS-NRPS | 100% | No new peak |
C14 | 29 kb | Terpene | 100% | No new peak |
C33 | 36 kb | type I PKS-NRPS | 100% | No new peak |
Streptomyces sp. NRRL F-525 | ||||
C10 | 50 kb | T1PKS,hglE-KS | 57% | No new peak |
C29 | 48 kb | NRPS-like | 100% | No new peak |
Streptomyces alni NRRL B-24611 | ||||
C7 | 59 kb | type II PKS | 100% | No new peak |
C11 | 46 kb | type II PKS | 100% | 4 new peaks |
C21 | 65 kb | type I PKS-NRPS | 100% | No new peak |
Streptomyces bicolor NRRL B-3897 | ||||
C6 | 47 kb | CDPS | 100% | No new peak |
C15 | 67 kb | type II PKS | 100% | 3 new peaks |
Streptomyces cyaneofuscatus NRRL B-2570 | ||||
C2 | 69 kb | type II PKS | 100% | 2 new peaks |
C24 | 43 kb | NRPS-like | 100% | No new peak |
Streptomyces griseofuscus NRRL B-5429 | ||||
C8 | 27 kb | type I PKS | 100% | No new peak |
C11 | 46 kb | type I PKS | 100% | No new peak |
C20 | 28 kb | type I PKS | 100% | No new peak |
C21 | 35 kb | type I PKS | 71% | No new peak |
C25 | 28 kb | type I PKS | 100% | 2 new peaks |
C38 | 25 kb | type I PKS | 100% | 3 new peaks |
Streptomyces peuceticus WC-3868 | ||||
C19 | 45 kb | type I PKS | 100% | No new peak |
Bacillus megaterium DSM 319 | ||||
C2 | 26 kb | Phosphonate | 100% | No new peak |
Bacillus megaterium QM B-1551 | ||||
C1 | 19 kb | Ladderane | 100% | No new peak |
C2 | 19 kb | Lanthipeptide | 100% | No new peak |
Bacillus thuringiensis YBT-020 | ||||
C1 | 14 kb | LAP | 100% | No new peak |
C2 | 14 kb | Arylpolyene | 100% | No new peak |
