Fig. 6: Study of fetal and postnatal development of Wistar and GK rats.
From: The Goto-Kakizaki rat is a spontaneous prototypical rodent model of polycystic ovary syndrome
a, b Body weight (a) and size (b) of 5 postnatal days (pnd) Wistar (W, open symbols) and GK (closed symbols) rats. c Representative histological sections of hematoxylin–eosin-stained ovaries of 5 pnd Wistar (left, n = 7) and GK (right, n = 5) rats. Black arrows: secondary follicles; dotted arrows: primary follicles; arrowheads: primordial follicles. Black dotted lines encircle the oocyte nest. Scale bars = 100 or 25 µm. d Serum AMH levels assessed on 5 pnd rats. e Anogenital distance (AGD) of 5 pnd rats. f, g Serum AMH (f) and blood glucose (g) levels measured before mating and at embryonic days (E) 13.5 and E20.5 of gestation on 2 month-old Wistar and GK rats. h, i Body weight (h) and size (i) of Wistar and GK rats fetuses at E18.5. j Ratio between body weight and placenta weight of E18.5 Wistar and GK rat fetuses. k Amhr2 (beige), Hsd3b1 (salmon), and Leptin (orange) mRNA levels in placenta of E18.5 GK rat fetuses, expressed as box with scatter blot with mean ± SEM (GK: n = 12; fold change relative to Wistar rats; W: n = 9). Unless indicated, data are represented as scatter blot with mean ± SEM. p-values are from two-sided Mann–Whitney U test for unpaired comparisons (GK vs. Wistar rats). n represents the number of biologically independent animals in each group. Source data are provided as a Source Data file.
