Fig. 2: PrimeDesign analysis of the ClinVar database.
From: PrimeDesign software for rapid and simplified design of prime editing guide RNAs
a The distribution of the number of designed pegRNA spacers per ClinVar variant. Candidate pegRNAs were determined based on the requirement of RTT length <35 nt and the RT extension to have a minimum homology of 5 nt downstream of the edit. b The 63,710 (91.7%) targetable ClinVar variants classified by type. The inner ring (gold) represents the proportion of targetable variants by type where at least one pegRNA could be designed to disrupt the PAM sequence (dark gold). The outer ring (gray) represents the proportion of targetable variants by type where at least one ngRNA could be designed for the PE3b strategy where the mismatch lies in the seed sequence (PAM-proximal nucleotides 1–10) (dark gray). See Supplementary Data 1 for details.
