Fig. 3: Installation of human pathogenic variants in HEK293T cells with PrimeDesign.
From: PrimeDesign software for rapid and simplified design of prime editing guide RNAs
a Overview of prime editing efficiencies for the installation of 20 human pathogenic variants in HEK293T cells, using PrimeDesign recommendations. Desired edit refers to sequencing reads containing only the edit of interest, while byproduct refers to sequencing reads containing any mutation(s) outside of only the edit of interest (i.e. indels, desired edit and indels). b Comparison between PE3 and PE3b editing strategies. c Assessing the effects of PAM-disrupting silent mutations on prime editing efficiencies. Mean ± s.d. of n = 3 independent biological replicates. Some of the data shown in a are also represented in b and c. See Supplementary Data 2 for details. Source Data is available in the Source Data file.
