Fig. 1: mESCs amplify the mouse template for ALT (mTALT) sequence to maintain telomere length.
From: Telomeres reforged with non-telomeric sequences in mouse embryonic stem cells

a Schematics of retrieving ALT mESCs. b Growth rate assay with crystal violet staining. (left) Representative images of each cell at daily intervals. (right) Quantified results. The bars represent means and SDs from ≥20 images per condition over five independent experiments. Scale bar, 20 μm. c Telomere length quantified from WGS. d TRF assay with DIG-(CCCTAA)*4 probe. Genomic DNA samples were cut using the indicated enzymes. S.M size marker. e Telomere length quantified from WGS according to read types. ‘Pair’ indicates that both read mates contain telomeric repeats, and ‘Single’ indicates that one read does. f Mapping result of the mate reads of single telomeric reads. g Snapshot of mTALT region with the mapped WGS data. h Calculated copy number of mTALT from WGS. Control, chr13:109,998,778-110,006,148. i Calculated copy number of mTALT from mmqPCR. mTALT-specific or telomere-specific protocols were used. The bars represent the means and SDs from three biologically independent replicates. j Representative images of fluorescence in situ hybridisation (FISH) of mTALT and telomeric repeats. Scale bar, 5 μm. k Quantification of mTALT and telomere co-localisation. ≥16 cells per condition were taken. Source data are provided as a Source Data file.