Fig. 4: Long non-coding RNA transcribed from ALT telomeres is important for telomere maintenance in ALT mESCs. | Nature Communications

Fig. 4: Long non-coding RNA transcribed from ALT telomeres is important for telomere maintenance in ALT mESCs.

From: Telomeres reforged with non-telomeric sequences in mouse embryonic stem cells

Fig. 4

a Copy number normalised ATAC-seq counts mapped to telomere and mTALT regions. b mTALT region snapshot of ATAC-seq peaks. c Quantification of ATAC-seq peaks mapped to specific peaks inside mTALT in b. d Snapshot of RNA-seq of the mTALT region. ‘Forward’ and ‘reverse’ denote the transcription directions. e Calculated levels of mTALT-containing (left) and telomeric repeat-containing transcripts (right) according to the transcriptional direction. f Snapshot of the SNPs of mTALT genomic sequences and transcripts. g Iso-Seq reads aligned to the mTALT region. The right ends of all reads are 5ʹ. I insulator. h Representative figure of the slot-blot with a telomere-specific probe for the DRIP assay. Input DNA was loaded at a 1/10 dilution. i Quantification of slot-blot results. The bars represent the means and SDs from three biologically independent replicates. P value from a two-tailed unpaired t-test: **P = 0.0012. j Representative images of interphase-TIF analysis. Scale bar, 5 μm. k Quantification of the ratio of cells with more than five TIFs. The bars represent the means and SDs from three biologically independent replicates. P values from a two-tailed unpaired t-test: **P = 0.0018. Source data are provided as a Source Data file.

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