Fig. 2: Profiling of human thymic epithelial cells at different stages. | Nature Communications

Fig. 2: Profiling of human thymic epithelial cells at different stages.

From: Single-cell transcriptional profiling of human thymic stroma uncovers novel cellular heterogeneity in the thymic medulla

Fig. 2

a UMAP visualization of epithelial cells colored by age group. b UMAP visualization of epithelial cells colored by cell types. c UMAP visualization of the expression of known marker genes used for cell cluster identification. d Heatmap showing the average expression of known marker genes in each epithelial cluster. e UMAP visualization of epithelial subsets in fetal, postnatal, and adult samples. f Immunofluorescence staining of AIRE+ (green) K5+ (red) mTEChi cells in postnatal and adult tissues. Arrowheads point to AIRE+ cells. Scale bars, 50 μm. g Thymic tissue from a 13-year-old donor was stained with CD8 (green) and CD4 (red) antibodies to visualize thymocytes together with a wide spectrum cytokeratin antibody to identify epithelial cells (blue). Scale bars, 50 μm. Staining in f and g was repeated twice with many donors with similar results.

Back to article page