Fig. 2: Notch2IC-expressing cells progressively repopulate the marginal zone.

a Immunofluorescence analysis of splenic sections from control and N2IC/creER^T2hom animals, with or without tamoxifen (TAM) treatment for the indicated time points. Sections were stained for FoB cells (IgD⁺, green), metallophilic macrophages (MOMA-1⁺, green), T cells (Thy1.2⁺, blue), and MZB cells (IgM⁺⁺, red). MZB cells are highlighted by white arrows, the marginal zone is additionally delineated with dotted white lines. b Chromogenic immunohistochemical analysis for hCD2 expression after tamoxifen treatments. N2IC/creER^T2hom mice were treated with 5 mg tamoxifen on 5 consecutive days. Indicated time points represent days after initial treatment. Notch2IC-expressing cells were stained for hCD2 (blue). The basement membranes of endothelial cells lining the MZ sinus and other blood vessels in the white and red pulp were stained by an anti-Laminin antibody (brown). The scale bars represent 100 µm. Images are representative for splenic sections of 2 mice per group.