Fig. 4: Notch2IC induces a transcriptomic shift from FoB to MZB signature.
From: Notch2-mediated plasticity between marginal zone and follicular B cells
Results of whole transcriptome RNA sequencing of 5-6 samples each of wild type FoB, MZB and hCD2+ and hCD2− N2IC/creERT2hom cells 3, 5, 7, and 14 days after tamoxifen (TAM) induction. a Unbiased multidimensional scaling (MDS) plot of 10% most variable genes across all sequenced samples. b Heatmap of all sequenced samples using predefined MZB signature gene sets (“FoB genes” and “MZB genes”) from control FoB and MZB cells as well as Notch2IC-expressing cells over time. Color scheme indicates log2-fold changes normalized across all samples. c Preranked GSEA analysis using the FoB and MZB signature gene sets. The ordered dataset displays 14450 genes, ranked for fold changes from upregulated (left) to downregulated (right) in hCD2+ versus hCD2− cells 14 days after TAM treatment. Enrichment scores are −0.87 for FoB gene set and 0.90 for MZB gene set (NOM p-values and FDR q-values < 10−3 for both).
