Fig. 6: FoB cells convert to MZB cells after adoptive transfer.

Flow cytometric analyses of surface phenotypes of recovered CD45.1⁺ cells in the spleen of recipient CD45.2⁺ mice at the indicated time points after adoptive transfer of purified FoB cells. a Representative plots of CD23/CD21 surface phenotype. Cells were gated on B220⁺ B cells and then separated for CD45.2⁺ recipient cells (black) or corresponding CD45.1⁺ recovered donor cells (red). The numbers indicate the percentages of MZB and FoB cells within CD45.1⁺ cells. b Representative overlays of an CD1d/CD23 surface staining of recovered CD45.1⁺ cells (red) and CD45.2⁺ recipient cells (gray) at the indicated time points after transplantation. The numbers indicate the percentages of MZB within CD45.1⁺ cells. c Summary and quantification of percentages of CD23^lowCD21^high MZB cells among CD45.1⁺ cells at different days after transfer. Bar graph shows mean values, SD and individual data points n = 4 (d1), n = 4 (d4), n = 2 (d9), n = 7 (d14) independent experiments. (*p = 0,0111, ****p = 1.0E−05, **p = 0,0093, *p = 0,0249, one-way ANOVA, Tukey´s multiple comparison test, only CD45.1⁺ cells are compared).