Fig. 7: FoB cells differentiate to MZB B cells via an MZP cell stage.

a Representative flow cytometric analysis of CD45.2⁺ recipient and CD45.1⁺ recovered donor cells for MZB precursor (MZP) and mature MZB cells at the indicated time points after adoptive transfer. Cells were gated for B220⁺ B cells, followed by identification of CD21^highIgM^high cells, comprising MZP and mature MZB cells. Plots are representative for n = 4 (d1), n = 4 (d4), n = 2 (d9), n = 7 (d14) independent experiments. b Colored histograms show downstream gating of CD21^highIgM^high for CD23 and CD1d levels to separate CD23^lowCD1d^high MZB from CD23⁺CD1d^med MZP cells. The gray histograms represent CD23 and CD1d expression from the CD21^intIgM^int FoB population. c Median fold changes in RNA levels of Cr2 (CD21), Cd1d (CD1d) and Fcer2a (CD23) in hCD2⁺ versus hCD2⁻cells over time after induction of Notch2IC-expression (experiment shown in Fig. 4).