Fig. 7: The proportion of Vangl2-replete cells neighbouring a deleted cell correlates with PNP eversion.
From: Cell non-autonomy amplifies disruption of neurulation by mosaic Vangl2 deletion in mice
a Illustrative examples of surface-subtracted neuroepithelia with low and high levels of EGFP recombination. Cell borders were determined from ZO1 staining. Green = Vangl2-deleted, blue = neighbour and white = distant cells. Scale bar = 50 µm. b Correlation between apical area and the number of neighbours (as predicted by Lewis’ law) of Vangl2-deleted cells. EGFP+ cells infrequently neighboured each other so total and Vangl2-replete (tdTom) cells were counted separately. Each dot represents a cell (234 cells from three embryos). c Positive correlation between the proportion of neuroepithelial cells which are EGFP+ and the proportion of EGFP− cells which neighbour an EGFP+ cell. Each dot represents an embryo. d Inverse correlation between the proportion of unique Vangl2-replete neighbours per Vangl2-deleted cell and overall % Cre-mediated recombination, demonstrating that non-autonomous effect amplification is limited by neighbour-sharing. e, f Correlation between neural fold eversion (negative elevation) in Cre;Fl− embryos and the proportion of cells which are Vangl2-deleted (e) or neighbours (f) in 15–20 somite stage embryos. The magenta box indicates the minimum degree of neural fold elevation observed in No Cre littermates. Each data point represents an individual embryo. p values and linear trend lines from Pearson correlation.
