Fig. 1: scNOMeRe-seq profiles in mouse preimplantation embryos.
From: Single-cell multiomics sequencing reveals the functional regulatory landscape of early embryos
a Schematic illustration of scNOMeRe-seq, including key steps, methods of library preparation, mouse preimplantation stages analyzed in this work and embryo numbers (n) outlined in the text. b Heat map showing copy number variations (CNV) inferred by DNA reads (left) and gene expression level (right, normalized each gene by average closest 100 genes) in mouse preimplantation blastomeres. Arrows indicate the examples of matched CNV inferences from DNA and RNA reads. c Bar plot showing the ratios of SNP tracked maternal DNA (top) or RNA (bottom) reads in total SNP tracked parental reads in each individual cell across preimplantation development. d Unsupervised clustering (left) and principle component analysis (right) of preimplantation blastomeres using gene expression level (top), DNA methylation level of 5 kilobases (kb) tiles (middle) and chromatin accessibility of all stage merged NDRs (bottom). n, the cell numbers of each dataset. e Profiles showing DNA methylation level (top), the weighted Pearson correlation coefficients of DNA methylation level vs gene expression level (bottom) along the gene bodies and 2.5 kb upstream of the transcription start sites (TSS) and 2.5 kb downstream of the transcription end sites (TES) of all genes for each stage. Met, WCG methylation; Expr, RNA expression. f Profiles showing chromatin accessibility (top), the weighted Pearson correlation coefficients of chromatin accessibility vs gene expression level (bottom), along the gene bodies and 2.5 kb upstream of the TSS and 2.5 kb downstream of the TES of all genes for each stage. Acc, GCH methylation. The sample size for b and c is provided in Supplementary Fig. 2a, and for d–f is provided in Supplementary Fig. 3g.
