Fig. 3: EVD survivor T cell memory response to EBOV glycoprotein GP1-4. | Nature Communications

Fig. 3: EVD survivor T cell memory response to EBOV glycoprotein GP1-4.

From: Characterisation of the T-cell response to Ebola virus glycoprotein amongst survivors of the 2013–16 West Africa epidemic

Fig. 3

IFNγ ELISpot using EVD survivor PBMC that were depleted of either CD4+ (−CD4) or CD8+ (−CD8) T cells. a Representative flow cytometry and ELISpot images for survivor C052 showing that PBMC were successfully depleted for the desired T cell population and the corresponding ELISpot response to the various stimulation conditions. b–d Response to GP1-4 peptide sub-pools. (b) shows the response to sub-pool 1 which consists of peptides 69–73, (c) shows the response to sub-pool 2 which contains peptides 74–80 and (d) shows the response to sub-pool 3 which contains peptides 81–88. Data show the mean +SD of 11 EVD survivor samples. One-way ANOVA with repeated measures used for statistical analysis in (c) p = 0.0264 and in (d) p = 0.0255, n = 11 biological independent samples examined over 2 independent experiments.

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