Fig. 2: Chemical mechanism of ubiquitination. | Nature Communications

Fig. 2: Chemical mechanism of ubiquitination.

From: RING domains act as both substrate and enzyme in a catalytic arrangement to drive self-anchored ubiquitination

Fig. 2

a Magnified regions of the active site of Ube2N~Ub/Ube2V2 (Ube2N in green, donor Ub in orange, acceptor Ub in red, and Ube2V2 in teal). Stereo-images are shown in Supplementary Fig. 1b. b Chemical scheme for the activation of the acceptor lysine. c Acid coefficients (pKa), d KM, and e kcat of di-ubiquitin formation by Ube2N/V2 are presented as best fit + standard error. Fits were performed using data of n = 3 technical replicates, and are shown in Supplementary Fig. 2 and Source data. Ub ubiquitin.

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