Fig. 2: TRAP labels orientation-selective V1 ensembles. | Nature Communications

Fig. 2: TRAP labels orientation-selective V1 ensembles.

From: Causal role for sleep-dependent reactivation of learning-activated sensory ensembles for fear memory consolidation

Fig. 2

a cfos::tdTom mice were presented with either a dark screen or an oriented grating (X°) and were then injected with tamoxifen prior to 3 days of housing in complete darkness. b, c Representative V1 tdTomato labeling quantified 11 days after tamoxifen administration. ****p = 0.0001 (t = 7.07, DF = 8) for dark screen vs. X°, nested t test (n = 5 mice/condition) Values in c indicate mean ± SEM. d Prior to tissue harvest, mice were either re-exposed to gratings of the same orientation (X°) or an alternate orientation (Y°). e, f Representative images showing overlap of tdTomato (red) and cFos protein (cyan). An example of colocalization within a neuron (quantified in f) is indicated with a white arrow for each image in the inset. **p = 0.009 (t = 3.22, DF = 10), nested t test (n = 5 mice for X°, n = 7 mice for Y°). Values in f indicate mean ± SEM. g, h Densities of tdTomato+ and cFos+ neurons were similar in X°- and Y°-exposed mice. Values indicate mean ± SEM.

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