Fig. 2: Decreased histamine H1R expression in cholinergic neurons induces sensorimotor gating ability deficit, social impairments, anhedonia-like behavior, and cognitive impairments.
a–l Sensorimotor gating, social behavior, hedonic function, and cognitive behavior in Hrh1fl/fl, ChAT-Cre, and ChAT-Cre;Hrh1fl/fl mice. a Percentage of prepulse inhibition of the auditory startle reflex across different prepulse intensities. b Percentage of prepulse inhibition of the auditory startle reflex across different prepulse intensities after treatment of antipsychotic agent risperidone, clozapine, or haloperidol. c Schematic diagram of three-chamber test and representative heat map images of phase 2 and phase 3. E: empty wire cage; S1: a gender-matched stranger mouse; S2: a new gender-matched stranger mouse. d Time in close interaction and preference index during sociability testing (phase 2) when exposed to S1. e Time in close interaction and preference index during subsequent social novelty recognition testing (phase 3) when exposed to S2 together with S1. f Time in close interaction and preference index of phase 2 in three-chamber test treated with antipsychotic agent risperidone, clozapine, or haloperidol. g Time in close interaction and preference index of phase 3 in three-chamber test treated with antipsychotic agent risperidone, clozapine, or haloperidol. h The representative images, percentage of the weight of cotton left unshredded, and nesting score in nest-building test. Scale bar, 2 cm. i Test for sucrose preference as a percentage of all fluid intake within a 48 h period. j Discrimination index in a novel object recognition test, with schematic diagram in the upper panel. k Discrimination index in temporal order memory test, with schematic diagram in the upper panel. l Percentage of correct choices in choice runs of delayed non-match-to-sample task. All data are presented as mean ± s.e.m. and error bars represent s.e.m. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ns nonsignificant. See also Supplementary Data 2 for further statistical information. Source data are provided as a Source Data file.
