Table 1 Calculated metal occupancies of COG0523 proteins in an idealised cella.

From: Calculating metalation in cells reveals CobW acquires CoII for vitamin B12 biosynthesis while related proteins prefer ZnII

Metal

MgIIGTP-CobW Eq. 2b

MgIIGTP-CobW Eq. 4c

MgIIGTP-YeiR Eq. 4c

MgIIGTPγS-YjiA Eq. 4c

MnII d

  

<0.8%

<1.8%

FeII d

<4.6%

<0.1%

<3.0%

< 3.4%

CoII

98.8%

91.9%

10.3%

2.0%

ZnII

86.2%

6.9%

24.4%

22.6%

NiII

0.1%

0.0%

0.0%

0.0%

CuI

0.5%

0.0%

0.2%

0.1%

Total

190.3%

98.9%

38.6%

29.8%

  1. aBased on metal availabilities in Salmonella under idealised conditions (ref. 9).
  2. bDoes not account for competition between different metals for the same high-affinity site in MgIIGTP-CobW.
  3. cTakes into account competition between multiple intracellular metals for the same site in each protein.
  4. dWhere only limiting metal–protein affinities (strongest KD limit) were determined, calculated occupancy corresponds to a maximum value (denoted by <).