Fig. 6: Compound 6g suppressed the LPS-induced inflammatory responses in RAW264.7 macrophages. | Nature Communications

Fig. 6: Compound 6g suppressed the LPS-induced inflammatory responses in RAW264.7 macrophages.

From: Marine furanocembranoids-inspired macrocycles enabled by Pd-catalyzed unactivated C(sp3)-H olefination mediated by donor/donor carbenes

Fig. 6

IC50: the concentration of the compound needed to inhibit inflammatory mediators by 50% relative to the control value. a Effect of 6g on LPS-induced TNF-α, IL-6, and IL-1β. Cells were treated with LPS alone (5 μg/mL) or with indicated concentrations of 6g for 24 h. b Effect of 6g on LPS-induced NF-κB activation. Dexamethasone was used as a positive control. IC50 values were calculated by nonlinear regression (curve fit) applied log (inhibitor) vs. normalized response. Data are shown as the mean ± SEM, n = biologically independent three wells for cytokine assays and two wells for Western blotting examined over two independent experiments.

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