Fig. 1: Characterization of the epigraph vaccine constructs. | Nature Communications

Fig. 1: Characterization of the epigraph vaccine constructs.

From: Epigraph hemagglutinin vaccine induces broad cross-reactive immunity against swine H3 influenza virus

Fig. 1

The three swH3 epigraph immunogens were computationally designed using the Epigraph vaccine designer tool to create a cocktail of immunogens designed to maximize potential epitope coverage in a population. The three epigraph hemagglutinin (HA) immunogens were aligned to the 1561 unique swine H3 HA sequences using a ClustalW alignment. A neighbor-joining tree was constructed to visualize the phylogenic relationship between the vaccine immunogens and the population of swH3 sequences. The three epigraph immunogens, the Texas/1998 (TX98) wild-type HA comparator, and the two FluSure strains are labeled for reference on the phylogenetic tree. The epigraph, wildtype, and FluSure vaccines are shown in the blue, green, and black boxes, respectively. The North American clusters, 2010 human-like lineage, and Eurasian lineage are circled in a dotted line (a). All three epigraph immunogens and the TX98 HA were cloned into a replication-defective Adenovirus type 5 (HAdV-5) vector and HA protein expression was confirmed by western blot. GAPDH is used as a cellular protein loading control (b). Confirmation of HA protein expression was obtained from three independent western blot experiments.

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