Fig. 2: Cross-reactive antibody responses with swH3 strains after vaccination in mice. | Nature Communications

Fig. 2: Cross-reactive antibody responses with swH3 strains after vaccination in mice.

From: Epigraph hemagglutinin vaccine induces broad cross-reactive immunity against swine H3 influenza virus

Fig. 2

BALB/c mice (n = 10) were vaccinated according to the timeline and vaccine dose (a). To examine the cross-reactivity of the antibody response after vaccination, a panel of 20 swH3 strains were selected that span the phylogenic tree. A maximum-likelihood tree was constructed to visualize the relationship between these assay strains and the vaccine immunogens (b). The cluster or lineage designation is in parentheses after the full strain name. Two weeks after boosting, mice were sacrificed, and sera were analyzed using a HI assay against the 20 swH3 representative strains (c). Cluster or lineage designation can be seen above the HI titer bars for each strain (one-way ANOVA with Tukey’s multiple comparisons compared to the epigraph group; n = 10 mice for all groups, except n = 5 for Nebraska/2013, Minnesota/2011, Ohio/2016, Denmark/2011, Italy/1985, Hong Kong/1976, Italy/1995). Data are presented as the mean with standard error (SEM). A heat map of these HI titers was constructed to further visualize the total cross-reactive antibody response of each vaccine.

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