Fig. 3: T-cell epitope mapping of four diverse swH3 strains after vaccination. | Nature Communications

Fig. 3: T-cell epitope mapping of four diverse swH3 strains after vaccination.

From: Epigraph hemagglutinin vaccine induces broad cross-reactive immunity against swine H3 influenza virus

Fig. 3

Splenocytes from vaccinated BALB/c mice (n = 5) were isolated and analyzed for cellular immunity using an IFNγ ELISpot. T-cell epitopes against the Ohio/2011, Manitoba/2005, Texas/1998, and Colorado/1977 strains were mapped using an overlapping peptide array consisting of 17-mers with 10-amino acid overlap which spanned the entire hemagglutinin (HA) protein. Peptide responses >50 spot-forming cells (SFC) per million were considered positive. Positive peptides for each vaccine and their location on the HA protein are indicated (a). The peptide number designates the position of the last amino acid in the peptide on the total HA protein. The level of response against each positive peptide is reported as SFC per million splenocytes with the dotted line indicating the 50 SFC/million cutoff (b). The total T-cell response to each virus peptide array is shown for all vaccination groups (n = 5; one-way ANOVA with Tukey’s multiple comparisons) (c). Data are presented as the mean with standard error (SEM).

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