Fig. 4: Protection against challenge with divergent swH3 viruses. | Nature Communications

Fig. 4: Protection against challenge with divergent swH3 viruses.

From: Epigraph hemagglutinin vaccine induces broad cross-reactive immunity against swine H3 influenza virus

Fig. 4

BALB/c mice (n = 10) were vaccinated with 1010 vp of HAdV-5-epigraph or HAdV-5-TX98, the commercial inactivated FluSure, or a PBS sham vaccine and challenged according to the timeline (a). An HI titer on mice sera was performed to examine the antibody response to the challenge virus strains after a single immunization (n = 10; one-way ANOVA with Tukey’s multiple comparisons compared to the epigraph group) (b). Mice were challenged intranasally (n = 10) with 104 TCID50 of Ohio/2011 (c), 105 TCID50 of Manitoba/2005 (d), or 103.5 TCID50 of Colorado/1977 (e) and monitored for weight loss over 14 days. Mice that reached 25% weight loss were humanely euthanized. Three days post infection, five mice per group were sacrificed to examine lung viral titers by TCID50 and qPCR (n = 5; one-way ANOVA with Tukey’s multiple comparisons compared to the epigraph group). Data are presented as the mean with standard error (SEM).

Back to article page