Fig. 5: Production of cytokines and activation of NF-κB and STAT3 are enhanced in Mettl3-null macrophages co-cultured with tumour cells.

a qRT-PCR analysis of Il-6 expression levels in B16 and LLC cells incubated with WT or KO BMDM culture medium for 24 hours in the presence or absence of BAY-11-7082 (5 µM) (n = 5 mice per group). b B16 and LLC cells were treated with TNF-α (40 ng/mL) or TNF-α + BAY-11-7082 (5 µM), followed by qRT-PCR analysis of Il-6. n = 3 independent experiments. c B16 and LLC cells co-cultured with WT or KO BMDMs in the presence of BAY-11-7082 (5 µM) or an anti-TNF-α Ab (20 µg/mL) for 8 h. qRT-PCR analysis of Il-6 expression levels in WT or KO BMDMs was performed (n = 5 mice per group). d Western blot analysis of the indicated proteins in WT and KO BMDMs co-cultured with B16 cells in the presence or absence of the anti-TNF-α Ab (20 µg/mL). The blots are representative of n = 3 independent experiments. e qRT-PCR analysis of Il-6 expression in WT and KO BMDMs co-cultured with B16 or LLC cells in the presence or absence of an anti-IL-6 Ab (20 µg/mL) (n = 5 mice per group). f Western blot analysis of the indicated proteins in WT and KO BMDMs co-cultured with B16 cells in the presence or absence of the anti-IL-6 Ab (20 µg/mL). The blots are representative of n = 3 independent experiments. Data are shown as the mean ± SD. P values were determined by a two-tailed t-test (a–c and e). *P ≤ 0.05, **P < 0.01 and ***P < 0.001. The source data are provided as a Source data file.