Fig. 3: PAF1C stabilizes the RNAPII–CSB interaction, but is not involved in TCR.
From: A CSB-PAF1C axis restores processive transcription elongation after DNA damage repair
a The left panel shows the Cryo-EM structure of RNAPII (in silver) bound to RAD26, which is the yeast orthologue of CSB, from the Wang lab (PDB-ID: 5VVR). The right panel shows the Cryo-EM structure of RNAPII (in silver) bound to PAF1C subunits (color-coded as indicated in the figure) from the Cramer lab (PDB-ID: 6GMH). b Co-immunoprecipitation of GFP-PAF1 in cells expressing either GFP-PAF1WT or GFP-PAF1ΔLEO1. c Co-immunoprecipitation of endogenous RNAPII-S2 in U2OS TIR1 cells or U2OS PAF-AID cells after depletion of PAF1 and at indicated timepoints after UV. d Validation of siRNA-mediated knockdown of CSB or PAF1 in XP168 LV cells by western blot analysis. Representative figure of two replicates. e TCR-UDS assay in XP-C primary fibroblasts (XP168LV) during 8 h following UV in cells transfected with the indicated siRNAs validated in d. Scale bar indicates 10 µm. f Quantification of TCR-UDS signal from e. Boxplots represent the median, 5th and 95th percentile of all cells of at least two independent experiments. b, c All co-immunoprecipitation figures are representative examples of at least two independent experiments.
