Fig. 1: Smn interacts with 7SK/hnRNP but not with 7SK/P-TEFb complexes.
From: Interaction of 7SK with the Smn complex modulates snRNP production
a Volcano plot of the 7SK interaction proteome following pulldown with Biotin-pd7SK and scr control from NSC-34 cell lysate. Smn complex proteins are highlighted in green, the 7SK core interactors Mepce and Larp7 are marked in red. b Western blot analysis of proteins co-immunoprecipitated from NSC-34 cells by an anti-Larp7 antibody directed against the C-terminus of Larp7. Immunoprecipitation with rabbit-IgG antibody was used as control. In, input; IP, immunoprecipitation. c Co-immunoprecipitation of proteins by an anti-Larp7 antibody directed against the N-terminal half of Larp7. d Co-immunoprecipitation of proteins by anti-Smn from NSC-34 cells. Lack of Gapdh co-precipitation serves as specificity control. e Sucrose gradient fractionation of NSC-34 cell lysate. Individual fractions were probed by Western blotting for proteins indicated on the right. Lower panel: Detection of 7SK in each fraction by qPCR. a.u., arbitrary units. f Co-immunoprecipitation of proteins by anti-SmB/B’ from NSC-34 cells. g qPCR analysis of 7SK co-precipitated by anti-SmB/B’ from NSC-34 cells. Data are mean ± standard deviation (s.d.); ***P ≤ 0.001; unpaired two-tailed t-test (n = 3). h Co-immunoprecipitation of proteins by anti-SmD1 from NSC-34 cells. Source data are provided as a Source Data file.
