Fig. 2: Enzymatic glycosylation on structure-switchable DNA-based glycan chip. | Nature Communications

Fig. 2: Enzymatic glycosylation on structure-switchable DNA-based glycan chip.

From: Glycan chip based on structure-switchable DNA linker for on-chip biosynthesis of cancer-associated complex glycans

Fig. 2

Scanned raw images and quantitative intensity plots for on-chip biosynthesized (a) Gb3 trisaccharide, (b) Gb4 tetrasaccharide, (c) Gb5 pentasaccharide, (d) SSEA-4 hexasaccharide, and (e) Globo H hexasaccharide. Synthesized complex glycans were detected by using biotinylated lectins and Alexa Fluor® 647-conjugated streptavidin. Each value presents the mean ± SEM from forty-nine independent spots excluding the highest and lowest signals. Statistical significance was assessed using Student’s unpaired t test (****p < 0.0001). GS-IB4 Griffonia simplicifolia isolectin B4, SBA soybean agglutinin lectin, RCA120 Ricinus communis agglutinin I lectin, MAL II Maackia amurensis lectin II, LTL Lotus tetragonolobus lectin, LgtC α-1,4-galactosyltransferase, LgtD β-1,3-N-acetylgalactosaminyltransferase/β-1,3-galactosyltransferase, α2,3-SialT α-2,3-sialyltransferase, α1,2-FucT α-1,2-fucosyltransferase. Symbols: blue circle, Glc; yellow circle, Gal; yellow square, GalNAc; red triangle, Fuc; purple square, Neu5Ac. Scale bar is 800 μm. Source data are provided as a Source Data file.

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