Fig. 3: SIESTA identified known and putative substrates for AKT1 kinase.
From: System-wide identification and prioritization of enzyme substrates by thermal analysis
a Scatterplot of Tm differences reveals the Tm shifts occurring only after simultaneous AKT1 + ATP addition (known and putative substrates are shown as green circles). b Representative melting curves for known and putative AKT1 substrates. c The reduction in the phosphorylation level of AKT1 substrate proteins by AKT1/2 inhibitor and ipatasertib treatment at 2.5 and 10 µM (all shown peptides are significant under all compound treatment conditions vs. DMSO condition (p < 0.05); n = 3 independent biological replicates, two-sided Student t-test). The phosphorylation levels of other phosphopeptides detected for the same proteins are compiled into one plot. N denotes the number of unchanged phosphopeptides for each protein. (Center line—median; box limits contain 50% of data; upper and lower quartiles, 75 and 25%; maximum—greatest value excluding outliers; minimum—least value excluding outliers; outliers—more than 1.5 times of the upper and lower quartiles) (AKT1 RAC-alpha serine/threonine-protein kinase or protein kinase B, ATP adenosine triphosphate, Tm melting temperature). Source data are provided as a Source Data file.
