Fig. 4: SIESTA identified and ranked known and novel PARP10 substrates.
From: System-wide identification and prioritization of enzyme substrates by thermal analysis
a Scatterplot of Tm differences reveals the shifts occurring when PARP10 + NAD are incubated with cell lysate. Known and putative substrates are shown in green circles. b Representative melting curves of putative PARP10 substrates. c Mono-ADP-ribosylation on a glutamic acid residue Glu110 in the PDRG1 peptide with the highest sequence-fitting score revealed by targeted ETD MS/MS of 3+ molecular ions (M). The fragments carrying the modification are marked with an asterisk (Tm melting temperature, NAD nicotinamide adenine dinucleotide, PARP10 poly-(ADP-ribose) polymerase-10). Source data are provided as a Source Data file.
