Fig. 5: TPP identifies protein interaction partners of enzymes added to cell lysates.
From: System-wide identification and prioritization of enzyme substrates by thermal analysis
a Proteins’ Tm shifting with addition of only enzyme to cell lysate. The known proteins interacting with AKT1 are shown in bold red (FUNCI, GET4, PHLPP2, and MAZ) (n = 2 independent biological replicates; two-sided Student t-test; no adjustment for multiple comparisons was performed). b Representative shifting proteins with each enzyme. DECR1 was also enriched in the affinity pulldown experiment with PARP10 (Tm melting temperature). Source data are provided as a Source Data file.
