Fig. 5: Sialic acids contribute to the differentiation of tumour moMac by interacting with Siglec-7 and Siglec-9 in monocytes.

A Presence of Siglec ligands in the supernatants of cell lines analysed by ELISA. B Histogram of Siglec-7 and Siglec-9 expression in circulating monocytes as analysed by flow cytometry, non-stained cells in grey and blood-derived monocytes from healthy donors in black line. C Analysis of CD163⁺ MR⁺ CD45⁺ CD14⁺ cells after the co-culture of tumour cell lines with monocytes in the presence of Siglec-7 and Siglec-9 blocking antibodies. Data presented as mean values ± SEM. Statistics: Friedman test (**p ≤ 0.01). D Representation of the role of CMAS in cellular sialylation. E Flow cytometric analysis of α2,3 linked sialic acids using MAAII lectin staining in the BxPC3 CMAS knockout and mock cells. F Expression of CD206 and CD163 in CD45⁺ CD14⁺ cells after co-culture of monocytes with mock and CMAS KO cell lines. Relative expression was calculated with respect to the mock cell lines (set as 1). Data presented as mean values ± SEM. Statistics: Two-tailed Wilcoxon test (*p < 0.05). G) IL-10 production after co-culture was analysed by ELISA. Data presented as mean values ± SEM. Statistics: Two-tailed Wilcoxon test (*p < 0.05).