Table 1 Blastocyst injection and germline transmission of ESCs.
Donor PSC | Prdm14 genotype in host embryo | No. (%) of injected embryos | No. (%) of pups | No. of live sex-matched chimeras | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
Cell type | Genotype | Gender | Transferred | Full-term | Analyzed | Chimeras | Analyzed | KO | GT | |
Rat ESC | Rosa26tdTomato/tdTomato | XY | KO or Hetero | 65 | 33 (51) | 31 | 24 (77) | 14 | 3 | 2 |
Rat ESC | Rosa26tdTomato/tdTomato | XX | KO or Hetero | 78 | 30 (38) | 30 | 15 (50) | 9 | 4 | 3 |
Rat ESC | Pax2/Pax8 KO | XY | KO or Hetero | 173 | 69 (40) | 67 | 33 (49) | 17 | 9 | 9 |
Rat ESC | Pax2/Pax8 KO | XX | KO or Hetero | 254 | 126 (50) | 126 | 37 (29) | 15 | 12 | 5b |
Rat ESC | MAC | XY | WT or Heteroa | 218 | 132 (61) | 132 | 112 (85) | 53 | – | 0 |
Rat ESC | MAC | XY | KO or Hetero | 22 | 18 (82) | 18 | 16 (89) | 7 | 5 | 5 |
Mouse ESC | Blimp1Tg(BAC-mEGFP)/+/CAG-nmScarlet | XY | KO or Hetero | 32 | 17 (53) | 17 | 9 (53) | 6 | 3 | 2 |
Mouse iPSC | CAG-EGFP | XY | KO or Hetero | 44 | 17 (39) | 17 | 9 (53) | 6 | 2 | 2 |
