Fig. 7: Human receptor mutations which disrupt the receptor–receptor interface.
From: Structures of mouse and human GITR–GITRL complexes reveal unique TNF superfamily interactions
a mIL-2 secretion upon hGITRL stimulation with hGITR cell lines expressing WT GITR, AA, and DD variants (n = 3; independent experiments performed three times). b WT cell line (top left) shows distinct puncta with fluorescently labeled hGITRL-sc trimer staining, while AA cell line (top right) shows reduced receptor binding and clustering. DD and RR (bottom panels) show near complete loss of receptor staining with hGITRL-sc trimers. This imaging experiment was performed once, cells plated in duplicate. Source data are provided as a Source Data file.
