Fig. 4: CD4+ TEM cell enrichment is associated with chronic activation and increased numbers of effector memory CD4+ T cells.
From: Virus-specific memory T cell responses unmasked by immune checkpoint blockade cause hepatitis
a–d Increased CD4+ TEM frequency before treatment reflects a decrease in circulating naïve CD4+ T cells and an increase in circulating numbers of CD4+ TEM cells. a Baseline naïve CD4+ T cell counts (n = 103; M.W.; Bonferroni correction: m = 4, p = 5.2 ⨯ 10−5). b Baseline CD4+ TEM cell counts (n = 103; M.W.; Bonferroni correction: m = 4, p = 8.0 ⨯ 10−4). c Baseline CD4+ TEMRA cell counts (n = 103; M.W.; Bonferroni-corrected p-value, m = 4). d Baseline CD4+ TCM cell counts (n = 103; M.W.; Bonferroni-corrected p-value, m = 4). Median values are indicated by a red line. e Pairwise correlations between CD4+ TEM cell frequency and other leucocyte subset frequencies in the training and validation sets (n = 103; Pearson). Example gating strategies for analysis of flow cytometry data are provided as Supplementary Figs. 2–8. f Baseline CD27+ CD4+ T cell frequencies (n = 103; M.W.; Bonferroni correction: m = 60, p = 4.2 ⨯ 10−10). g Baseline CD57+ CD4+ T cell frequencies (n = 103; M.W.; Bonferroni correction: m = 60, p = 2.0 ⨯ 10−9). h Baseline HLA-DR+ CD4+ T cell frequencies (n = 103; M.W.; Bonferroni correction: m = 60, p = 4.1 ⨯ 10−5). i Baseline CD279+ CD4+ T cell frequencies (n = 103; M.W.; Bonferroni-corrected p-value, m = 60). j Baseline CD160+ CD244+ CD8+ T cell frequencies (n = 103; M.W.; Bonferroni-corrected p-value, m = 60). k Baseline Torque Teno Virus (TTV) loads. Uninfected patients were censored from analysis (n = 74; M.W.; Bonferroni-corrected p-value, m = 60). Median values are indicated by a red line.
