Table 1 Kinetic parameters of AdhP and AldDHb.

From: Hyperproduction of 3-hydroxypropionate by Halomonas bluephagenesis

Enzymes

Km (mM)

Kcat (s−1)

Kcat/Km (s−1 mM−1)

AdhPa

35.41 ± 1.525

4.11 ± 0.05

0.12 ± 0.004

AdhPb

1.75 ± 0.278

19.39 ± 0.62

11.19 ± 1.304

AldDHbc

0.05 ± 0.002

3.38 ± 0.09

67.45 ± 0.797

  1. Each value represents the mean ± standard deviation in triplicate experiments.
  2. aAssay conditions: 100 mM potassium carbonate buffer (pH 9.0) containing 30 mM ammonium sulfate; 2 mM, NAD+; 9.7 μg mL−1, AdhP; 1,3-propanediol concentration was varied from 1.17 to 600 mM; temperature was set at 37 °C.
  3. bAssay conditions: 50 mM Tris-HCl buffer (pH 7.0) containing 0.1 mM NADH; 0.36 μg mL−1, AdhP; 3HPA concentration was varied from 0.01 to 20 mM; temperature was set at 37 °C.
  4. cAssay conditions: 50 mM, potassium phosphate buffer (pH 8.0) containing 1.0 mM DTT; 2 mM, NAD+; 5.7 μg mL−1, AldDHb; 3HPA concentration was varied from 0.02 to 15 mM; temperature was set at 37 °C.
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