Fig. 3: mHTT inhibits protein synthesis in vitro. | Nature Communications

Fig. 3: mHTT inhibits protein synthesis in vitro.

From: Mutant Huntingtin stalls ribosomes and represses protein synthesis in a cellular model of Huntington disease

Fig. 3

A Coomassie gel showing FL-wtHTT 23Q (250 ng) and FL-mHTT 48Q (250 ng), n = 3 independent experiments. B Shows the percentage of relative luciferase unit (RLU) in presence of an indicated concentration of recombinant FL-wtHTT and FL-mHTT. HTT proteins normalized to the maximum luminescence in BSA (75 μM). Data are mean ± SEM (n = 3 independent experiments). ****P < 0.0001 by one-way ANOVA followed by Tukey’s multiple comparison test. ##P < 0.01 by two-tailed Student’s t test. C In vitro translation (IVT) assay in presence of different concentrations of recombinant FL-wtHTT 23Q and FL-mHTT 48Q or BSA. Bar graphs depict the percentage of relative luciferase unit (RLU) normalized to the maximum luminescence in BSA (83 nM). Data are mean ± SEM (n = 3 independent experiments). ***P < 0.001, ****P < 0.0001 by one-way ANOVA followed by Bonferroni’s multiple comparisons test. D Shows luciferase activity at 15, 45, and 75 min of IVT reaction with 150 nM recombinant FL-wtHTT 23Q and FL-mHTT 48Q proteins or BSA (500 nM). Data are mean ± SEM (n = 4 independent experiments). *P < 0.05, **P < 0.01, ***P < 0.001, by one-way ANOVA followed by Tukey’s multiple comparison test. Exact P values are reported in the Source Data file. Source data are provided as a Source Data file.

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