Fig. 6: Dual-color imaging of two vesicle membrane cargos and super-resolution imaging with pHmScarlet.
From: pHmScarlet is a pH-sensitive red fluorescent protein to monitor exocytosis docking and fusion steps
a TIRF images of INS-1 cells expressing VAMP2-SEP and Syp-pHmScarlet. The locations of vesicle fusion are indicated by yellow rectangles, scale bar: 5 µm. Experiments were repeated three times. b Time-lapses of the marked exocytotic events (bottom, 1.3 × 1.3 µm), scale bars: 0.5 μm. c Normalized intensity traces of the marked event in the green and red channels of the same vesicle. d Wide-field TIRF image and e montages of a representative fusion event with ring structure in a VAPM2-pHmScarlet-labeled vesicle (magenta). Experiments were repeated five times for (d) and (e). f Intensity traces of the marked event shown in (e).
