Fig. 4: Effect of fermentation conditions on CS sulfation.
From: Complete biosynthesis of a sulfated chondroitin in Escherichia coli

a Schematic showing growth and cultivation of CS-producing E. coli strains. b Effect of Induction OD600 on CS titer and sulfation in K4ΔkfoEΔcysH(DE3)pETM6-Sw. c CS sulfation obtained from different inducer concentrations and expression temperatures for 0.6 and 1.0 OD600 induction points. Error bars represent standard deviation from three biological replicates. Source data underlying Figs. 4b and c are provided as a Source Data file.