Fig. 5: Effect of export on CS sulfation. | Nature Communications

Fig. 5: Effect of export on CS sulfation.

From: Complete biosynthesis of a sulfated chondroitin in Escherichia coli

Fig. 5

a Representation of currently accepted CPS transport complex in E. coli producing group 2 capsules. b Repression of GAG transport using CRISPRi in K4ΔkfoEΔcysH(DE3)pETM6-Sw showing 2.5-fold decreased GAG export. c CS sulfation in transport repressed K4ΔkfoEΔcysH(DE3) expressing Sw with uninduced and unrepressed controls. Error bars represent standard deviation from three biological replicates. Source data underlying Figs. 5b and c are provided as a Source Data file.

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