Fig. 2: Analysis of blood myeloid cells shows unique features associated with patient status and outcome.

a Expression heatmap of the 10 clusters identified among the blood neutrophils. b Two-dimensional UMAP embedding of the blood neutrophil. Cells are colored according to their cluster. c Scatter plot of the Correspondence Analysis (CA) of the blood neutrophil populations. d Proportion of resting neutrophils (left panel), ISGs neutrophils (middle panel) and immature neutrophils (right panel) among blood neutrophils according to patient clinical status. A one-sided Tukey’s range test was used in the left panel while Kruskal–Wallis rank test was used in both middle and right panel. Median and 5–95% theoretical quantiles are shown. Normality of the distribution in the left panel was assessed using a Shapiro–Wilk test in each group individually (p = 0.39, p = 0.99, and p = 0.043, respectively). N = 32 independent clinical samples were used, including 5 derived from healthy patients, 6 from mild patients, and 21 from severe patients. e T-cell suppression ability of CD14⁺ monocytes according to clinical status (right) or to ICU outcome (severe patients only, right panel). A Tukey’s range test was used in the left panel while a two-sided Welch’s t test was performed in the right panel (t = 4.9 with a degree of freedom equal to 11.8). Median and 5–95% theoretical quantiles are shown. Normality of the distribution in the right panel was assessed using a Shapiro–Wilk test in each group individually (p=0.91 and p=0.7 respectively). In the left panel, N = 29 independent clinical samples were used, including 4 derived from healthy patients, 7 from mild patients and 18 from severe patients. In the right panel, N = 18 independent clinical samples were used, including 11 derived from patients who survived and 7 from deceased patients. f Association between CD14⁺ monocytes ARG1 Mean Fluorescence Intensity (MFI) and immunesuppression. The dashed line corresponds to the fitted Hill-like function. N = 29 independent clinical samples were used, including 4 derived from healthy patients, 7 from mild patients and 18 from severe patients. g Association between CD14⁺ monocytes HLA-DR Mean Fluorescence Intensity (MFI) and immunesuppression. Source data are provided as a Source Data file.