Fig. 1: Conventional RNA-seq identifies differentially expressed genes (DEGs) in E14.5 Atoh7-null, Pou4f2-null, and Isl1-null retinas.

a Clustering of all DEGs across all four genotypes based on Z scores indicates the Atoh7-null retina is least similar, whereas the Isl1-null retina is most similar to the wild-type retina. Each genotype has three replicate samples. The genes were divided into five groups (1–5) based on how they are affected in the three mutants. The color scale represents cross-sample Z scores for individual genes (rows). Source data are from Supplementary Data 2. b A Venn-diagram showing the overlaps of downregulated genes in all three mutant retinas. c A Venn-diagram showing the overlaps of upregulated genes in the three mutant retinas. Source data for (b, c) are provided.