Fig. 2: Synthetic strategy leading to monofunctionalized molecular tweezers.
From: Specific inhibition of the Survivin–CRM1 interaction by peptide-modified molecular tweezers
a Introduction of one butynyl phosphate ester arm on the parent diacetoxy tweezer (TCA coupling) followed by click chemistry with N-terminal azidopeptide; b additional introduction of a fluorescence label by adding a C-terminal LysFAM-Gly sequence to the clicked peptide. Blue: phosphate; red: alkyne/triazole; green: azide/triazole; olive: FAM label. DIPEA N,N-diisopropylethylamine, THF tetrahydrofuran.
