Fig. 4: NMR chemical shift perturbation and signal intensity analyses allow us to map binding of molecular tweezers to Survivin120.
From: Specific inhibition of the Survivin–CRM1 interaction by peptide-modified molecular tweezers
NMR intensity changes and signal shifts of Survivin120 in complex with one equivalent of tweezers compared to Survivin alone plotted against the amino acid sequence. Normalized signal intensities as well as signal shifts for the unmodified tweezer TW (a, b), TW-ELTL (c, d), and TW-ELTLGEFL (e, f) were identified for each signal and plotted against the Survivin sequence (residues 2-117 as assigned in the BMRB data-base). Residues that were excluded from analysis because they are not visible in the spectra or suffer from signal overlap are marked with an asterisk. Residues with a prominent shift or reduced signal intensity (red) are clustered around Survivin’s NES, and lysine and arginine residues are additionally marked with an arrow. Upon titration with TW-ELTL and TW-ELTLGEFL, signal intensities collapse around the NES region. Source data are provided as a Source Data file.
