Fig. 3: RBD-contacting glycans in the RaTG13 and PCoV_GX spikes. | Nature Communications

Fig. 3: RBD-contacting glycans in the RaTG13 and PCoV_GX spikes.

From: Bat and pangolin coronavirus spike glycoprotein structures provide insights into SARS-CoV-2 evolution

Fig. 3

a Detailed structures of the RBD-glycans interface are shown. The RaTG13 RBD is colored in magenta, PCoV_GX RBD in green, SARS-CoV-2 (PDB ID: 6VXX) RBD in wheat, and SARS-CoV-2 (PDB ID: 6ZGE) RBD in marine; remaining regions shown in gray. Glycans are shown as red sticks and labeled. Sequence alignment of the SARS-CoV-2, RaTG13 and PCoV_GX RBD-contacting glycosylation sites is shown in the bottom panel. Sequences between the three sites are omitted and indicated by black dots. Amino acid positions of asparagines are indicated above the sequences according to SARS-CoV-2. Asparagines (N) are colored red and threonines (T) are colored blue. b Cryo-EM analysis of the RaTG13_T372A and negative-staining EM analysis of the PCoV_GX_T370A. The representative micrographs, 2D classification and 3D classification results were shown.

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