Fig. 4: Binding of linoleic acid (LA) with the PCoV_GX spike.

a LA-bound PCoV_GX spike shown in side view and top view. The LA is shown as orange spheres. b LA in the pocket of PCoV_GX spike RBD. LA is shown as orange sticks with EM density and PCoV_GX spike is colored green. LA-interacting amino acid residues are shown as sticks. c LC-MS analysis of extracted lipids from the purified PCoV_GX spike from two independent experiments. The chemical structure and molecular weight of LA are shown in the top panel, C18 column elution profile in the middle panel, and the molecular weight of corresponding peak in the bottom panel.